![]() ![]() We hope these protocols will be helpful to you in your research. Then we import this data into Prism software (GraphPad) to conduct further data analysis. ATS uses SOFTMax Pro software connected to a plate reader to determine the A490 value. This protocol will give an example of how to process the data from a Cytotoxicity Assay. This protocol should help you design and execute appropriately. There are many factors that go into a successful cytotoxicity assay. ![]() It takes five days to complete this assay. This protocol includes photos of what your plates should look like during the assay process. There is also a link to an Online Calculator.Ĭytotoxicity Assay for Targeted Toxins in vitro. This protocol will help in determining the correct amount of material to use in your assay. #IT-27) with your biotinylated targeting agent (peptide, ligand, cytokine, growth factor, antibody, etc.).Ĭoncentration Calculation: Convert molarity to mg/ml and mg/ml to molarity. This protocol will be helpful when using our streptavidin-saporin conjugate (Streptavidin-ZAP, Cat. Preparing for a Cytotoxicity Assay using Streptavidin-ZAP. This protocol will be helpful when using our secondary antibody-saporin conjugates with your primary antibody. Preparing for a Cytotoxicity Assay using Secondary Conjugates. Protocols to assist in preparing for, executing and interpreting results are now posted on our website. One of the tests you can use to test your targeting agent for internalization is the in vitro Cytotoxicity Assay. ![]()
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